Detection of genomic deletions in rice using oligonucleotide microarrays.

ABSTRACT:

Background

The induction of genomic deletions by physical- or chemical- agents is an easy and inexpensive means to generate a genome-saturating collection of mutations. Different mutagens can be selected to ensure a mutant collection with a range of deletion sizes. This would allow identification of mutations in single genes or, alternatively, a deleted group of genes that might collectively govern a trait (e.g., quantitative trait loci, QTL). However, deletion mutants have not been widely used in functional genomics, because the mutated genes are not tagged and therefore, difficult to identify. Here, we present a microarray-based approach to identify deleted genomic regions in rice mutants selected from a large collection generated by gamma ray or fast neutron treatment. Our study

SUBMITTER: Bruce M 

PROVIDER: S-EPMC2666768 | biostudies-literature | 2009 Mar

REPOSITORIES: biostudies-literature