Identification and validation of reference genes for quantitative RT-PCR normalization in wheat.

ABSTRACT:

Background

Usually the reference genes used in gene expression analysis have been chosen for their known or suspected housekeeping roles, however the variation observed in most of them hinders their effective use. The assessed lack of validated reference genes emphasizes the importance of a systematic study for their identification. For selecting candidate reference genes we have developed a simple in silico method based on the data publicly available in the wheat databases Unigene and TIGR.

Results

The expression stability of 32 genes was assessed by qRT-PCR using a set of cDNAs from 24 different plant samples, which included different tissues, developmental stages and temperature stresses. The selected sequences included 12 well-known HKGs representing different functional

SUBMITTER: Paolacci AR 

PROVIDER: S-EPMC2667184 | biostudies-literature | 2009 Feb

REPOSITORIES: biostudies-literature