Construction of a taste-blind medaka fish and quantitative assay of its preference-aversion behavior.

ABSTRACT: In vertebrates, the taste system provides information used in the regulation of food ingestion. In mammals, each cell group within the taste buds expresses either the T1R or the T2R taste receptor for preference-aversion discrimination. However, no such information is available regarding fish. We developed a novel system for quantitatively assaying taste preference-aversion in medaka fish. In this study, we prepared fluorescently labeled foods with fine cavities designed to retain tastants until they were bitten by the fish. The subjects were fed food containing a mixture of amino acids and inosine monophosphate (AN food), denatonium benzoate (DN food) or no tastant (NT food), and the amounts of ingested food were measured by fluorescence microscopy. Statistical analysis of the fluorescenc

SUBMITTER: Aihara Y 

PROVIDER: S-EPMC2667311 | biostudies-literature | 2008 Nov

REPOSITORIES: biostudies-literature