Analysis of cell surface proteome changes via label-free, quantitative mass spectrometry.

ABSTRACT: We present a mass spectrometry-based strategy for the specific detection and quantification of cell surface proteome changes. The method is based on the label-free quantification of peptide patterns acquired by high mass accuracy mass spectrometry using new software tools and the cell surface capturing technology that selectively enriches glycopeptides exposed to the cell exterior. The method was applied to monitor dynamic protein changes in the cell surface glycoproteome of Drosophila melanogaster cells. The results led to the construction of a cell surface glycoprotein atlas consisting of 202 cell surface glycoproteins of D. melanogaster Kc167 cells and indicated relative quantitative changes of cell surface glycoproteins in four different cellular states. Furthermore we specifically inv

SUBMITTER: Schiess R 

PROVIDER: S-EPMC2667347 | biostudies-literature | 2009 Apr

REPOSITORIES: biostudies-literature