MRMaid, the web-based tool for designing multiple reaction monitoring (MRM) transitions.

ABSTRACT: Multiple reaction monitoring (MRM) of peptides uses tandem mass spectrometry to quantify selected proteins of interest, such as those previously identified in differential studies. Using this technique, the specificity of precursor to product transitions is harnessed for quantitative analysis of multiple proteins in a single sample. The design of transitions is critical for the success of MRM experiments, but predicting signal intensity of peptides and fragmentation patterns ab initio is challenging given existing methods. The tool presented here, MRMaid (pronounced "mermaid") offers a novel alternative for rapid design of MRM transitions for the proteomics researcher. The program uses a combination of knowledge of the properties of optimal MRM transitions taken from expert practitioners a

SUBMITTER: Mead JA 

PROVIDER: S-EPMC2667351 | biostudies-literature | 2009 Apr

REPOSITORIES: biostudies-literature