Functional differentiation of Brassica napus guard cells and mesophyll cells revealed by comparative proteomics.

ABSTRACT: Guard cells are highly specialized cells that form tiny pores called stomata on the leaf surface. The opening and closing of stomata control leaf gas exchange and water transpiration as well as allow plants to quickly respond and adjust to new environmental conditions. Mesophyll cells are specialized for photosynthesis. Despite the phenotypic and obvious functional differences between the two types of cells, the full protein components and their functions have not been explored but are addressed here through a global comparative proteomics analysis of purified guard cells and mesophyll cells. With the use of isobaric tags for relative and absolute quantification (iTRAQ) tagging and two-dimensional liquid chromatography mass spectrometry, we identified 1458 non-redundant proteins in both gu

SUBMITTER: Zhu M 

PROVIDER: S-EPMC2667361 | biostudies-literature | 2009 Apr

REPOSITORIES: biostudies-literature