Sensitive and specific identification of wild type and variant proteins from 8 to 669 kDa using top-down mass spectrometry.

ABSTRACT: Top-down and bottom-up mass spectrometry methods can generate gas phase fragments and use these to identify proteins. Top-down methods, in addition, can provide the mass of the protein itself and therefore additional structural information. Despite the conceptual advantage of top-down methods, the market share advantage belongs to bottom-up methods as a result of their more robust sample preparation, fragmentation, and data processing methods. Here we report improved fragmentation and data processing methods for top-down mass spectrometry. Specifically we report the use of funnel-skimmer dissociation, a variation of nozzle-skimmer dissociation, and compare its performance with electron capture dissociation. We also debut BIG Mascot, an extended version of Mascot with incorporated top-down

SUBMITTER: Karabacak NM 

PROVIDER: S-EPMC2667363 | biostudies-literature | 2009 Apr

REPOSITORIES: biostudies-literature