Increased expression of heat shock protein 105 in rat uterus of early pregnancy and its significance in embryo implantation.

ABSTRACT:

Background

Heat shock proteins (Hsps) are a set of highly conserved proteins, Hsp105, has been suggested to play a role in reproduction.

Methods

Spatio-temporal expression of Hsp105 in rat uterus during peri-implantation period was examined by immunohistochemistry and Western blot, pseudopregnant uterus was used as control. Injection of antisense oligodeoxynucleotides to Hsp105 into pregnant rat uteri was carried out to look at effect of Hsp105 on embryo implantation.

Results

Expression of Hsp105 was mainly in the luminal epithelium on day 1 of pregnancy, and reached a peak level on day 5, whereas in stroma cells, adjacent to the implanting embryo, the strongest expression of Hsp105 was observed on day 6. The immunostaining profile in the uterus was consistent with th

SUBMITTER: Yuan JX 

PROVIDER: S-EPMC2667524 | biostudies-literature | 2009 Mar

REPOSITORIES: biostudies-literature