Digital PCR provides sensitive and absolute calibration for high throughput sequencing.

ABSTRACT:

Background

Next-generation DNA sequencing on the 454, Solexa, and SOLiD platforms requires absolute calibration of the number of molecules to be sequenced. This requirement has two unfavorable consequences. First, large amounts of sample-typically micrograms-are needed for library preparation, thereby limiting the scope of samples which can be sequenced. For many applications, including metagenomics and the sequencing of ancient, forensic, and clinical samples, the quantity of input DNA can be critically limiting. Second, each library requires a titration sequencing run, thereby increasing the cost and lowering the throughput of sequencing.

Results

We demonstrate the use of digital PCR to accurately quantify 454 and Solexa sequencing libraries, enabling the preparation of se

SUBMITTER: White RA 

PROVIDER: S-EPMC2667538 | biostudies-literature | 2009 Mar

REPOSITORIES: biostudies-literature