Determination of enriched histone modifications in non-genic portions of the human genome.

ABSTRACT:

Background

Chromatin immunoprecipitation followed by high-throughput sequencing (ChIP-seq) has recently been used to identify the modification patterns for the methylation and acetylation of many different histone tails in genes and enhancers.

Results

We have extended the analysis of histone modifications to gene deserts, pericentromeres and subtelomeres. Using data from human CD4+ T cells, we have found that each of these non-genic regions has a particular profile of histone modifications that distinguish it from the other non-coding regions. Different methylation states of H4K20, H3K9 and H3K27 were found to be enriched in each region relative to the other regions. These findings indicate that non-genic regions of the genome are variable with respect to histone modificatio

SUBMITTER: Rosenfeld JA 

PROVIDER: S-EPMC2667539 | biostudies-literature | 2009 Mar

REPOSITORIES: biostudies-literature