Identification of bdm-1, a gene involved in G protein beta-subunit function and alpha-subunit accumulation.

ABSTRACT: Targeted disruption of Galpha and Gbeta genes has established the requirement of an intact G protein signaling pathway for optimal execution of several important physiological processes, including pathogenesis, in the chestnut blight fungus Cryphonectria parasitica. We now report the identification of a G protein signal transduction component, beta disruption mimic factor-1, BDM-1. Disruption of the corresponding gene, bdm-1, resulted in a phenotype indistinguishable from that previously observed after disruption of the Gbeta subunit gene, cpgb-1. The BDM-1 deduced amino acid sequence contained several significant clusters of identity with mammalian phosducin, including a domain corresponding to a highly conserved 11-amino acid stretch that has been implicated in binding to the Gbetagamma

SUBMITTER: Kasahara S 

PROVIDER: S-EPMC26677 | biostudies-literature | 2000 Jan

REPOSITORIES: biostudies-literature