Investigation of fatty acid elongation and desaturation steps in Fusarium lateritium by quantitative two-dimensional deuterium NMR spectroscopy in chiral oriented media.

ABSTRACT: The origin of hydrogen atoms during fatty acid biosynthesis in Fusarium lateritium has been quantified by isotope tracking close to natural abundance. Methyl linoleate was isolated from F. lateritium grown in natural abundance medium or in medium slightly enriched with labeled water, glucose, or acetate, and the (2)H incorporation was determined by quantitative (2)H-{(1)H} NMR in isotropic and chiral oriented solvents. Thus, the individual ((2)H/(1)H)(i) ratio at each pro-R and pro-S hydrogen position of the CH(2) groups along the chain can be analyzed. These values allow the isotope redistribution coefficients (a(ij)) that characterize the specific source of each hydrogen atom to be related to the nonexchangeable hydrogen atoms in glucose and to the medium water. In turn, these can be rel

SUBMITTER: Baillif V 

PROVIDER: S-EPMC2667766 | biostudies-literature | 2009 Apr

REPOSITORIES: biostudies-literature