Toll-like receptor 5 engagement modulates tumor development and growth in a mouse xenograft model of human colon cancer.

ABSTRACT:

Background & aims

Toll-like receptor (TLR)-dependent signaling was proposed as immunotherapeutic targets against invading pathogens and tumorigenesis. Here, we investigated whether TLR5-dependent signaling modulates colonic tumor development in mouse xenograft model of human colon cancer.

Methods

The expression of myeloid differentiation factor 88 (MyD88) or TLR5 was stably knocked down in human colon cancer cells (DLD-1). Nude mice were subcutaneously implanted with MyD88-knocked down (KD), TLR5-KD, or control cells (n = 16) to examine the pathophysiology of tumor xenografts. Protein microarray assessed the differential expression of cytokines in these tumors. Leukocyte infiltration and tumor angiogenesis were assessed by immunohistochemistry with antibodies against neutrop

SUBMITTER: Rhee SH 

PROVIDER: S-EPMC2667819 | biostudies-literature | 2008 Aug

REPOSITORIES: biostudies-literature