Use of gene expression profiles in cells of peripheral blood to identify new molecular markers of acute pancreatitis.

ABSTRACT:

Hypothesis

Blood leukocytes play a major role in mediating local and systemic inflammation during acute pancreatitis. We hypothesize that peripheral blood mononuclear cells (PBMCs) in circulation exhibit unique changes in gene expression and could provide a "reporter" function that reflects the inflammatory response in the pancreas with acute pancreatitis.

Design

To determine specific changes in blood leukocytes during acute pancreatitis, we studied the gene transcription profile in PBMCs in a rat model of experimental pancreatitis (sodium taurocholate). Normal rats, saline controls, and a model of septic shock were used as a controls. Complementary RNA obtained from PBMCs of each group (n = 3 in each group) were applied to Affymetrix rat genome DNA GeneChip arrays. Main Out

SUBMITTER: Bluth M 

PROVIDER: S-EPMC2668141 | biostudies-literature | 2008 Mar

REPOSITORIES: biostudies-literature