Quantitative analysis of cell cycle phase durations and PC12 differentiation using fluorescent biosensors.

ABSTRACT: Cell cycle analysis typically relies on fixed time-point measurements of cells in particular phases of the cell cycle. The cell cycle, however, is a dynamic process whose subtle shifts are lost by fixed time-point methods. Live-cell fluorescent biosensors and time-lapse microscopy allows the collection of temporal information about real time cell cycle progression and arrest. Using two genetically-encoded biosensors, we measured the precision of the G(1), S, G(2) and M cell cycle phase durations in different cell types and identified a bimodal G(1) phase duration in a fibroblast cell line that is not present in the other cell types. Using a cell line model for neuronal differentiation, we demonstrated that NGF-induced neurite extension occurs independently of NGF-induced cell cycle G(1) ph

SUBMITTER: Hahn AT 

PROVIDER: S-EPMC2668240 | biostudies-literature | 2009 Apr

REPOSITORIES: biostudies-literature