Species-specific identification of a wide range of clinically relevant fungal pathogens by use of Luminex xMAP technology.

ABSTRACT: In immunocompromised patients suffering from invasive fungal infection, rapid identification of the fungal species is a prerequisite for selection of the most appropriate antifungal treatment. We present an assay permitting reliable identification of a wide range of clinically relevant fungal pathogens based on the high-throughput Luminex microbead hybridization technology. The internal transcribed spacer (ITS2) region, which is highly variable among genomes of individual fungal species, was used to generate oligonucleotide hybridization probes for specific identification. The spectrum of pathogenic fungi covered by the assay includes the most commonly occurring species of the genera Aspergillus and Candida and a number of important emerging fungi, such as Cryptococcus, Fusarium, Trichospo

SUBMITTER: Landlinger C 

PROVIDER: S-EPMC2668305 | biostudies-literature | 2009 Apr

REPOSITORIES: biostudies-literature