MiRNA expression profiles in head and neck squamous cell carcinoma and adjacent normal tissue.

ABSTRACT:

Background

The expression of miRNA in head and neck squamous cell carcinomas (HNSCCs) that had been classified as high risk by surgical pathologic features and validated by trial outcome for disease recurrence was determined and compared with matched adjacent normal tissues.

Methods

miRNA and corresponding gene expression were determined using miRNA bioarrays and gene expression arrays.

Results

Twenty miRNAs were determined to be differentially regulated in the HNSCC samples when compared with their normal tissue counterparts. Quantitative reverse transcriptase-polymerase chain reaction confirmed differential regulation of miRNA expression, and gene expression analysis on these same-paired samples confirmed the loss of putative mRNA targets including genes such as ade

SUBMITTER: Ramdas L 

PROVIDER: S-EPMC2668741 | biostudies-literature | 2009 May

REPOSITORIES: biostudies-literature