Protein kinase C in porcine retinal arteries and neuroretina following retinal ischemia-reperfusion.

ABSTRACT:

Purpose

Identification of the intracellular signal-transduction pathways activated in retinal ischemia may be important in revealing novel pharmacological targets. To date, most studies have focused on identifying neuroprotective agents. The retinal blood vessels are key organs in circulatory failure, and this study was therefore designed to examine the retinal vasculature separately from the neuroretina.

Methods

Retinal ischemia was induced by elevating the intraocular pressure in porcine eyes, followed by 5, 12, or 20 h of reperfusion. Protein kinase C (PKC)alpha, PKCbeta1, and PKCbeta2 mRNA levels, and protein expression were determined using real-time PCR, western blot, and immunofluorescence staining techniques.

Results

The retinal arteries could easily be dissec

SUBMITTER: Gesslein B 

PROVIDER: S-EPMC2668768 | biostudies-literature | 2009

REPOSITORIES: biostudies-literature