Diffuse-type gastric carcinoma: progression, angiogenesis, and transforming growth factor beta signaling.

ABSTRACT:

Background

Diffuse-type gastric carcinoma is a cancer with poor prognosis that has high levels of transforming growth factor beta (TGF-beta) expression and thick stromal fibrosis. However, the association of TGF-beta signaling with diffuse-type gastric carcinoma has not been investigated in detail.

Methods

We used a lentiviral infection system to express a dominant-negative TGF-beta type II receptor (dnTbetaRII) or green fluorescent protein (GFP) as a control in the diffuse-type gastric carcinoma cell lines, OCUM-2MLN and OCUM-12. These infected cells and the corresponding parental control cells were subcutaneously or orthotopically injected into nude mice. Angiogenesis was inhibited by infecting cells with a lentivirus carrying the gene for angiogenic inhibitor thrombospond

SUBMITTER: Komuro A 

PROVIDER: S-EPMC2669102 | biostudies-literature | 2009 Apr

REPOSITORIES: biostudies-literature