Analysis of all protein phosphatase genes in Aspergillus nidulans identifies a new mitotic regulator, fcp1.

ABSTRACT: Reversible protein phosphorylation is an important regulatory mechanism of cell cycle control in which protein phosphatases counteract the activities of protein kinases. In Aspergillus nidulans, 28 protein phosphatase catalytic subunit genes were identified. Systematic deletion analysis identified four essential phosphatases and four required for normal growth. Conditional alleles of these were generated using the alcA promoter. The deleted phosphatase strain collection and regulatable versions of the essential and near-essential phosphatases provide an important resource for further analysis of the role of reversible protein phosphorylation to the biology of A. nidulans. We further demonstrate that nimT and bimG have essential functions required for mitotic progression since their deletio

SUBMITTER: Son S 

PROVIDER: S-EPMC2669206 | biostudies-literature | 2009 Apr

REPOSITORIES: biostudies-literature