Pro370Leu mutant myocilin disturbs the endoplasm reticulum stress response and mitochondrial membrane potential in human trabecular meshwork cells.

ABSTRACT:

Purpose

To investigate the impact of Pro370Leu mutant myocilin on endoplasmic reticulum (ER) stress response and mitochondria function in human trabecular meshwork (HTM) cells.

Methods

HTM cells were transfected with wild-type Pro370Leu mutant myocilin or pcDNA3.1 (+) expression plasmids. The effect of the mutant myocilin on ER stress response was semiquantitatively evaluated by determining the expression level of 78 kDa glucose-regulated protein (GRP78) using reverse transcription-polymerase chain reaction and phosphorylation of the alpha subunit of eukaryotic translation initiation factor 2 (eIF2alpha) using western blot analysis. Mitochondria function was determined by analyzing the changes in mitochondrial membrane potential (Deltapsim), measured by flow cytometry analys

SUBMITTER: Wang L 

PROVIDER: S-EPMC2669509 | biostudies-literature | 2007 Apr

REPOSITORIES: biostudies-literature