Site-specific integration of adeno-associated virus involves partial duplication of the target locus.

ABSTRACT: A variety of viruses establish latency by integrating their genome into the host genome. The integration event generally occurs in a nonspecific manner, precluding the prediction of functional consequences from resulting disruptions of affected host genes. The nonpathogenic adeno-associated virus (AAV) is unique in its ability to stably integrate in a site-specific manner into the human MBS85 gene. To gain a better understanding of the integration mechanism and the consequences of MBS85 disruption, we analyzed the molecular structure of AAV integrants in various latently infected human cell lines. Our study led to the observation that AAV integration causes an extensive but partial duplication of the target gene. Intriguingly, the molecular organization of the integrant leaves the possibil

SUBMITTER: Henckaerts E 

PROVIDER: S-EPMC2669786 | biostudies-literature | 2009 May

REPOSITORIES: biostudies-literature