MicroRNA expression ratio is predictive of head and neck squamous cell carcinoma.

ABSTRACT:

Purpose

The involvement of microRNAs in cancer and their potential as biomarkers of diagnosis and prognosis are becoming increasingly appreciated. We sought to identify microRNAs altered in head and neck squamous cell carcinoma (HNSCC) and to determine whether microRNA expression is predictive of disease.

Experimental design

RNA isolated from fresh-frozen primary tumors, fresh-frozen nondiseased head and neck epithelial tissues, and HNSCC cell lines was profiled for the expression of 662 microRNAs by microarray. The microRNAs that were both differentially expressed on the array and by quantitative reverse transcription-PCR were subsequently validated by quantitative reverse transcription-PCR using a total of 99 HNSCC samples and 14 normal epithelia.

Results

A marked d

SUBMITTER: Avissar M 

PROVIDER: S-EPMC2669849 | biostudies-literature | 2009 Apr

REPOSITORIES: biostudies-literature