Stable kinetochore-microtubule interactions depend on the Ska complex and its new component Ska3/C13Orf3.

ABSTRACT: Ska1 and Ska2 form a complex at the kinetochore-microtubule (KT-MT) interface and are required for timely progression from metaphase to anaphase. Here, we use mass spectrometry to search for additional components of the Ska complex. We identify C13Orf3 (now termed Ska3) as a novel member of this complex and map the interaction domains among the three known components. Ska3 displays similar characteristics as Ska1 and Ska2: it localizes to the spindle and KT throughout mitosis and its depletion markedly delays anaphase transition. Interestingly, a more complete removal of the Ska complex by concomitant depletion of Ska1 and Ska3 results in a chromosome congression failure followed by cell death. This severe phenotype reflects a destabilization of KT-MT interactions, as demonstrated by reduc

SUBMITTER: Gaitanos TN 

PROVIDER: S-EPMC2669960 | biostudies-literature | 2009 May

REPOSITORIES: biostudies-literature