Shift from apoptotic to necrotic cell death during human papillomavirus-induced transformation of keratinocytes.

ABSTRACT: Oncogenic transformation is a complex, multistep process, which goes through several stages before complete malignant transformation occurs. To identify early processes in carcinogenesis, we used an in vitro model, based on the initiating event in cervical cancer, papillomavirus transformation of keratinocytes. We compared gene expression in primary keratinocytes (K) and papillomavirus-transformed keratinocytes from early (E) and late (L) passages and from benzo[a]pyrene-treated L cells (BP). The transformed cells exhibit similar transcriptional changes to clinical cervical carcinoma. The number of transcripts expressed progressively decreased during the evolution from K to BP cells. Bioinformatic analysis, validated by detailed biochemical analysis, revealed substantial contraction of bot

SUBMITTER: Kravchenko-Balasha N 

PROVIDER: S-EPMC2670175 | biostudies-literature | 2009 Apr

REPOSITORIES: biostudies-literature