Interaction and colocalization of CaBP4 and Unc119 (MRG4) in photoreceptors.

ABSTRACT:

Purpose

To characterize the interaction of the neuron-specific protein CaBP4 with the synaptic photoreceptor protein Unc119 homolog (MRG4).

Methods

The interaction of CaBP4 and Unc119 was studied using affinity chromatography, yeast two-hybrid system, coimmunoprecipitation, and gel overlay assay. The colocalization of CaBP4 and Unc119 was analyzed using immunohistochemistry. Unc119, CaBP4, and synaptic proteins were examined in photoreceptors using immunohistochemistry and in synaptic tangential sections of flatmounted frozen retinas using Western blot analysis.

Results

Biochemical evidence supported the interaction of CaBP4 with Unc119. CaBP4 and Unc119 colocalized in the photoreceptor synapse of adult retina and during postnatal retinal development. A reduction in U

SUBMITTER: Haeseleer F 

PROVIDER: S-EPMC2670247 | biostudies-literature | 2008 Jun

REPOSITORIES: biostudies-literature