Spatiotemporal association of DNAJB13 with the annulus during mouse sperm flagellum development.

ABSTRACT:

Background

The sperm annulus is a septin-based fibrous ring structure connecting the midpiece and the principal piece of the mammalian sperm flagellum. Although ultrastructural abnormalities and functional importance of the annulus have been addressed in Sept4-null mutant mice and a subset of human patients with asthenospermia syndrome, little is known about how the structure is assembled and positioned to the midpiece-principal piece junction during mammalian sperm flagellum development.

Results

By performing immunofluorescence and biochemical approaches with antibodies against DNAJB13 and an annulus constituent SEPT4, we report here a spatiotemporal association of DNAJB13 with sperm annulus during mouse sperm flagellum development. DNAJB13 co-localized with SEPT4 to the an

SUBMITTER: Guan J 

PROVIDER: S-EPMC2670831 | biostudies-literature | 2009 Mar

REPOSITORIES: biostudies-literature