MRM screening/biomarker discovery with linear ion trap MS: a library of human cancer-specific peptides.

ABSTRACT:

Background

The discovery of novel protein biomarkers is essential in the clinical setting to enable early disease diagnosis and increase survivability rates. To facilitate differential expression analysis and biomarker discovery, a variety of tandem mass spectrometry (MS/MS)-based protein profiling techniques have been developed. For achieving sensitive detection and accurate quantitation, targeted MS screening approaches, such as multiple reaction monitoring (MRM), have been implemented.

Methods

MCF-7 breast cancer protein cellular extracts were analyzed by 2D-strong cation exchange (SCX)/reversed phase liquid chromatography (RPLC) separations interfaced to linear ion trap MS detection. MS data were interpreted with the Sequest-based Bioworks software (Thermo Electron). In-

SUBMITTER: Yang X 

PROVIDER: S-EPMC2670839 | biostudies-literature | 2009 Mar

REPOSITORIES: biostudies-literature