Transgenic overexpression of a stable Plasminogen Activator Inhibitor-1 variant.

ABSTRACT:

Introduction

Plasminogen Activator Inhibitor-1 (PAI-1) is a member of the Serine Protease Inhibitor (SERPIN) gene family and a key regulator of fibrinolysis. PAI-1 is unique among SERPINs in its spontaneous transition to a latent, inactive state, with a half-life of approximately 2 hours under physiologic conditions. The biologic importance of the PAI-1 transition to latency is unknown. This study aimed to engineer transgenic overexpression of a stable murine PAI-1 variant to examine the physiologic effects in vivo from delayed transition of PAI-1 to latency.

Materials and methods

Ten independent transgenic lines were generated with expression of a stable PAI-1 variant driven by the hybrid CMV/chicken beta-actin promoter.

Results

Plasma PAI-1 levels in the transgenic

SUBMITTER: Fahim AT 

PROVIDER: S-EPMC2670886 | biostudies-literature | 2009 Mar

REPOSITORIES: biostudies-literature