Using triplet periodicity of nucleotide sequences for finding potential reading frame shifts in genes.

ABSTRACT: We introduce a novel approach for the detection of possible mutations leading to a reading frame (RF) shift in a gene. Deletions and insertions of DNA coding regions are considerable events for genes because an RF shift results in modifications of the extensive region of amino acid sequence coded by a gene. The suggested method is based on the phenomenon of triplet periodicity (TP) in coding regions of genes and its relative resistance to substitutions in DNA sequence. We attempted to extend 326 933 regions of continuous TP found in genes from the KEGG databank by considering possible insertions and deletions. We revealed totally 824 genes where such extension was possible and statistically significant. Then we generated amino acid sequences according to active (KEGG's) and hypothetically

SUBMITTER: Frenkel FE 

PROVIDER: S-EPMC2671204 | biostudies-literature | 2009 Apr

REPOSITORIES: biostudies-literature