Quantitative genome-scale analysis of protein localization in an asymmetric bacterium.

ABSTRACT: Despite the importance of subcellular localization for cellular activities, the lack of high-throughput, high-resolution imaging and quantitation methodologies has limited genomic localization analysis to a small number of archival studies focused on C-terminal fluorescent protein fusions. Here, we develop a high-throughput pipeline for generating, imaging, and quantitating fluorescent protein fusions that we use for the quantitative genomic assessment of the distributions of both N- and C-terminal fluorescent protein fusions. We identify nearly 300 localized Caulobacter crescentus proteins, up to 10-fold more than were previously characterized. The localized proteins tend to be involved in spatially or temporally dynamic processes and proteins that function together and often localize tog

SUBMITTER: Werner JN 

PROVIDER: S-EPMC2671984 | biostudies-literature | 2009 May

REPOSITORIES: biostudies-literature