Proteomic studies of a single CNS synapse type: the parallel fiber/purkinje cell synapse.

ABSTRACT: Precise neuronal networks underlie normal brain function and require distinct classes of synaptic connections. Although it has been shown that certain individual proteins can localize to different classes of synapses, the biochemical composition of specific synapse types is not known. Here, we have used a combination of genetically engineered mice, affinity purification, and mass spectrometry to profile proteins at parallel fiber/Purkinje cell synapses. We identify approximately 60 candidate postsynaptic proteins that can be classified into 11 functional categories. Proteins involved in phospholipid metabolism and signaling, such as the protein kinase MRCKgamma, are major unrecognized components of this synapse type. We demonstrate that MRCKgamma can modulate maturation of dendritic spines

SUBMITTER: Selimi F 

PROVIDER: S-EPMC2672601 | biostudies-literature | 2009 Apr

REPOSITORIES: biostudies-literature