Application of proteomics in the discovery of candidate protein biomarkers in a diabetes autoantibody standardization program sample subset.

ABSTRACT: Novel biomarkers of type 1 diabetes must be identified and validated in initial, exploratory studies before they can be assessed in proficiency evaluations. Currently, untargeted "-omics" approaches are underutilized in profiling studies of clinical samples. This report describes the evaluation of capillary liquid chromatography (LC) coupled with mass spectrometry (MS) in a pilot proteomic analysis of human plasma and serum from a subset of control and type 1 diabetic individuals enrolled in the Diabetes Autoantibody Standardization Program, with the goal of identifying candidate biomarkers of type 1 diabetes. Initial high-resolution capillary LC-MS/MS experiments were performed to augment an existing plasma peptide database, while subsequent LC-FTICR studies identified quantitative differ

SUBMITTER: Metz TO 

PROVIDER: S-EPMC2672959 | biostudies-literature | 2008 Feb

REPOSITORIES: biostudies-literature