Learning/memory impairment and reduced expression of the HNK-1 carbohydrate in beta4-galactosyltransferase-II-deficient mice.

ABSTRACT: The glycosylation of glycoproteins and glycolipids is important for central nervous system development and function. Although the roles of several carbohydrate epitopes in the central nervous system, including polysialic acid, the human natural killer-1 (HNK-1) carbohydrate, alpha2,3-sialic acid, and oligomannosides, have been investigated, those of the glycan backbone structures, such as Galbeta1-4GlcNAc and Galbeta1-3GlcNAc, are not fully examined. Here we report the generation of mice deficient in beta4-galactosyltransferase-II (beta4GalT-II). This galactosyltransferase transfers Gal from UDP-Gal to a nonreducing terminal GlcNAc to synthesize the Gal beta1-4GlcNAc structure, and it is strongly expressed in the central nervous system. In behavioral tests, the beta4GalT-II(-/-) mice showe

SUBMITTER: Yoshihara T 

PROVIDER: S-EPMC2673321 | biostudies-literature | 2009 May

REPOSITORIES: biostudies-literature