Integrated proteomic and transcriptomic profiling of mouse lung development and Nmyc target genes.

ABSTRACT: Although microarray analysis has provided information regarding the dynamics of gene expression during development of the mouse lung, no extensive correlations have been made to the levels of corresponding protein products. Here, we present a global survey of protein expression during mouse lung organogenesis from embryonic day E13.5 until adulthood using gel-free two-dimensional liquid chromatography coupled to shotgun tandem mass spectrometry (MudPIT). Mathematical modeling of the proteomic profiles with parallel DNA microarray data identified large groups of gene products with statistically significant correlation or divergence in coregulation of protein and transcript levels during lung development. We also present an integrative analysis of mRNA and protein expression in Nmyc loss- an

SUBMITTER: Cox B 

PROVIDER: S-EPMC2673710 | biostudies-literature | 2007

REPOSITORIES: biostudies-literature