Glutamine increases autophagy under Basal and stressed conditions in intestinal epithelial cells.

ABSTRACT:

Background & aims

Glutamine plays a protective role in intestinal cells during physiologic stress; however, the protection mechanisms are not fully understood. Autophagy functions in bulk degradation of cellular components, but has been recognized recently as an important mechanism for cell survival under conditions of stress. We therefore sought to see if glutamine's actions involve the induction of autophagy in intestinal cells and, if so, the mechanisms that underlie this action.

Methods

Formation of microtubule-associated protein light chain 3 (LC3)-phospholipid conjugates (LC3-II) in rat intestinal epithelial IEC-18 cells and human colonic epithelial Caco-2(BBE) cells was determined by Western blotting and localized by confocal microscopy. Activation of mammalian target

SUBMITTER: Sakiyama T 

PROVIDER: S-EPMC2673957 | biostudies-literature | 2009 Mar

REPOSITORIES: biostudies-literature