HAC stability in murine cells is influenced by nuclear localization and chromatin organization.

ABSTRACT:

Background

Human artificial chromosomes (HAC) are small functional extrachromosomal elements, which segregate correctly during each cell division. In human cells, they are mitotically stable, however when the HAC are transferred to murine cells they show an increased and variable rate of loss. In some cell lines the HAC are lost over a short period of time, while in others the HAC become stable without acquiring murine DNA.

Results

In this study, we linked the loss rate to the position of the HAC in the murine cell nucleus with respect to the chromocenters. HAC that associated preferentially with the chromocenter displayed a lower loss rate compared to the HAC that are less frequently associated. The chromocenter acts as a hub for the deposition of heterochromatic markers, c

SUBMITTER: Moralli D 

PROVIDER: S-EPMC2674426 | biostudies-literature | 2009 Mar

REPOSITORIES: biostudies-literature