Searching for novel cell cycle regulators in Trypanosoma brucei with an RNA interference screen.

ABSTRACT:

Background

The protozoan parasite, Trypanosoma brucei, is spread by the tsetse fly and causes Human African Trypanosomiasis. Its cell cycle is complex and not fully understood at the molecular level. The T. brucei genome contains over 6000 protein coding genes with >50% having no predicted function. A small scale RNA interference (RNAi) screen was carried out in Trypanosoma brucei to evaluate the prospects for identifying novel cycle regulators.

Results

Procyclic form T. brucei were transfected with a genomic RNAi library and 204 clones isolated. However, only 76 RNAi clones were found to target a protein coding gene of potential interest. These clones were screened for defects in proliferation and cell cycle progression following RNAi induction. Sixteen clones exhibited pro

SUBMITTER: Monnerat S 

PROVIDER: S-EPMC2674452 | biostudies-literature | 2009 Mar

REPOSITORIES: biostudies-literature