Placental gene expression responses to maternal protein restriction in the mouse.

ABSTRACT:

Objective

Maternal protein restriction has been shown to have deleterious effects on placental development, and has long-term consequences for the progeny. We tested the hypothesis that, by the use of microarray technology, we could identify specific genes and cellular pathways in the developing placenta that are responsive to maternal protein deprivation, and propose a potential mechanism for observed gene expression changes.

Methods

We fed pregnant FVB/NJ mice from day post-coitum 10.5 (DPC10.5) to DPC17.5, an isocaloric diet containing 50% less protein than normal chow. We used the Affymetrix Mouse 430A_2.0 array to measure gene expression changes in the placenta. We functionally annotated the regulated genes, and examined over-represented functional categories and perfor

SUBMITTER: Gheorghe CP 

PROVIDER: S-EPMC2674533 | biostudies-literature | 2009 May

REPOSITORIES: biostudies-literature