Ontology highlight
ABSTRACT:
SUBMITTER: Doyon Y
PROVIDER: S-EPMC2674762 | biostudies-literature | 2008 Jun
REPOSITORIES: biostudies-literature
Nature biotechnology 20080525 6
We describe the use of zinc-finger nucleases (ZFNs) for somatic and germline disruption of genes in zebrafish (Danio rerio), in which targeted mutagenesis was previously intractable. ZFNs induce a targeted double-strand break in the genome that is repaired to generate small insertions and deletions. We designed ZFNs targeting the zebrafish golden and no tail/Brachyury (ntl) genes and developed a budding yeast-based assay to identify the most active ZFNs for use in vivo. Injection of ZFN-encoding ...[more]