The F(0)F(1)-ATP synthase complex contains novel subunits and is essential for procyclic Trypanosoma brucei.

ABSTRACT: The mitochondrial F(0)F(1) ATP synthase is an essential multi-subunit protein complex in the vast majority of eukaryotes but little is known about its composition and role in Trypanosoma brucei, an early diverged eukaryotic pathogen. We purified the F(0)F(1) ATP synthase by a combination of affinity purification, immunoprecipitation and blue-native gel electrophoresis and characterized its composition and function. We identified 22 proteins of which five are related to F(1) subunits, three to F(0) subunits, and 14 which have no obvious homology to proteins outside the kinetoplastids. RNAi silencing of expression of the F(1) alpha subunit or either of the two novel proteins showed that they are each essential for the viability of procyclic (insect stage) cells and are important for the stru

SUBMITTER: Zikova A 

PROVIDER: S-EPMC2674945 | biostudies-literature | 2009 May

REPOSITORIES: biostudies-literature