Screening a genome-wide S. pombe deletion library identifies novel genes and pathways involved in genome stability maintenance.

ABSTRACT: The maintenance of genome stability is essential for an organism to avoid cell death and cancer. Based on screens for mutant sensitivity against DNA damaging agents a large number of DNA repair and DNA damage checkpoint genes have previously been identified in genetically amenable model organisms. These screens have however not been exhaustive and various genes have been, and remain to be, identified by other means. We therefore screened a genome-wide Schizosaccharomyces pombe deletion library for mutants sensitive against various DNA damaging agents. Screening the library on different concentrations of these genotoxins allowed us to assign a semi-quantitative score to each mutant expressing the degree of sensitivity. We isolated a total of 229 mutants which show sensitivity to one or more

SUBMITTER: Deshpande GP 

PROVIDER: S-EPMC2675035 | biostudies-literature | 2009 May

REPOSITORIES: biostudies-literature