Effect of freezing on PCR amplification of 16S rRNA genes from microbes associated with black band disease of corals.

ABSTRACT: Molecular analysis of black band disease of corals revealed that samples frozen immediately after collection yielded more proteobacterial 16S rRNA sequences, while unfrozen samples produced more cyanobacterial and sulfur-oxidizing bacterial sequences. These results suggest the need to use multiple approaches for preparation of samples to characterize this complex polymicrobial disease.

SUBMITTER: Sekar R 

PROVIDER: S-EPMC2675240 | biostudies-literature | 2009 Apr

REPOSITORIES: biostudies-literature