Crystallization of human complement component C3b in the presence of a staphylococcal complement-inhibitor protein (SCIN).

ABSTRACT: Staphylococcus aureus secretes a number of small proteins that effectively attenuate the human innate immune response. Among these, the staphylococcal complement-inhibitor protein (SCIN) disrupts the function of the complement component 3 (C3) convertase that is initiated through either the classical or the alternative pathway and thereby prevents amplification of the complement response on the bacterial surface. Recent studies have shown that SCIN may affect the activities of the C3 convertase by binding in an equimolar fashion to C3b, which is itself an integral although non-enzymatic component of the convertase. In order to better understand the nature of the C3b-SCIN interaction, the hanging-drop vapor-diffusion technique was used to crystallize human C3b in the presence of a recombina

SUBMITTER: Garcia BL 

PROVIDER: S-EPMC2675590 | biostudies-literature | 2009 May

REPOSITORIES: biostudies-literature