Purification, crystallization and preliminary X-ray diffraction analysis of a plant subtilase.

ABSTRACT: The subtilase SBT3 from Solanum lycopersicum (tomato) was purified from a tomato cell culture and crystallized using the sitting-drop vapour-diffusion method. A native data set was collected to 2.5 A resolution at 100 K using synchrotron radiation. For experimental phasing, CsCl-derivative and tetrakis(acetoxymercuri)methane (TAMM) derivative crystals were employed for MIRAS phasing. Three caesium sites and one TAMM site were identified, which allowed solution of the structure.

SUBMITTER: Rose R 

PROVIDER: S-EPMC2675601 | biostudies-literature | 2009 May

REPOSITORIES: biostudies-literature