Direct sampling from human liver tissue cross sections for electrophoretic analysis of doxorubicin.

ABSTRACT: After chemoembolization of the liver with doxorubicin (Dox), this drug and its metabolites are not homogeneously distributed in this organ. The distribution cannot be easily measured making it difficult to assess how the drug performs in different tissue regions. Here we report a technique for sampling tissue cross sections that can analyze the contents of micrometer size regions. The tissue cross sections were from the explanted liver of a hepatocellular carcinoma patient. Samples were directly aspirated from a 5 microm thick tissue cross section into a 50 microm i.d. capillary where the tissue was solubilized with a separation buffer containing sodium dodecyl sulfate. Upon sample dissolution, Dox and natively fluorescent compounds were separated and detected by micellar electrokinetic ch

SUBMITTER: Wang Y 

PROVIDER: S-EPMC2675659 | biostudies-literature | 2009 May

REPOSITORIES: biostudies-literature