Proteomic discovery of previously unannotated, rapidly evolving seminal fluid genes in Drosophila.

ABSTRACT: As genomic sequences become easier to acquire, shotgun proteomics will play an increasingly important role in genome annotation. With proteomics, researchers can confirm and revise existing genome annotations and discover completely new genes. Proteomic-based de novo gene discovery should be especially useful for sets of genes with characteristics that make them difficult to predict with gene-finding algorithms. Here, we report the proteomic discovery of 19 previously unannotated genes encoding seminal fluid proteins (Sfps) that are transferred from males to females during mating in Drosophila. Using bioinformatics, we detected putative orthologs of these genes, as well as 19 others detected by the same method in a previous study, across several related species. Gene expression analysis re

SUBMITTER: Findlay GD 

PROVIDER: S-EPMC2675977 | biostudies-literature | 2009 May

REPOSITORIES: biostudies-literature