Reduced expression of CD45 protein-tyrosine phosphatase provides protection against anthrax pathogenesis.

ABSTRACT: The modulation of cellular processes by small molecule inhibitors, gene inactivation, or targeted knockdown strategies combined with phenotypic screens are powerful approaches to delineate complex cellular pathways and to identify key players involved in disease pathogenesis. Using chemical genetic screening, we tested a library of known phosphatase inhibitors and identified several compounds that protected Bacillus anthracis infected macrophages from cell death. The most potent compound was assayed against a panel of sixteen different phosphatases of which CD45 was found to be most sensitive to inhibition. Testing of a known CD45 inhibitor and antisense phosphorodiamidate morpholino oligomers targeting CD45 also protected B. anthracis-infected macrophages from cell death. However, reduced

SUBMITTER: Panchal RG 

PROVIDER: S-EPMC2676018 | biostudies-literature | 2009 May

REPOSITORIES: biostudies-literature