Budding yeast centrosome duplication requires stabilization of Spc29 via Mps1-mediated phosphorylation.

ABSTRACT: Protein phosphorylation plays an important role in the regulation of centrosome duplication. In budding yeast, numerous lines of evidence suggest a requirement for multiple phosphorylation events on individual components of the centrosome to ensure their proper assembly and function. Here, we report the first example of a single phosphorylation event on a component of the yeast centrosome, or spindle pole body (SPB), that is required for SPB duplication and cell viability. This phosphorylation event is on the essential SPB component Spc29 at a conserved Thr residue, Thr(240). Mutation of Thr(240) to Ala is lethal at normal gene dosage, but an increased copy number of this mutant allele results in a conditional phenotype. Phosphorylation of Thr(240) was found to promote the stability of the

SUBMITTER: Holinger EP 

PROVIDER: S-EPMC2676026 | biostudies-literature | 2009 May

REPOSITORIES: biostudies-literature