Osteogenic differentiation of mesenchymal stem cells promoted by overexpression of connective tissue growth factor.

ABSTRACT:

Objective

Large segmental bone defect repair remains a clinical and scientific challenge with increasing interest focusing on combining gene transfection with tissue engineering techniques. The aim of this study is to investigate the effect of connective tissue growth factor (CTGF) on the proliferation and osteogenic differentiation of the bone marrow mesenchymal stem cells (MSCs).

Methods

A CTGF-expressing plasmid (pCTGF) was constructed and transfected into MSCs. Then expressions of bone morphogenesis-related genes, proliferation rate, alkaline phosphatase activity, and mineralization were examined to evaluate the osteogenic potential of the CTGF gene-modified MSCs.

Results

Overexpression of CTGF was confirmed in pCTGF-MSCs. pCTGF transfection significantly enhanced

SUBMITTER: Wang JJ 

PROVIDER: S-EPMC2676415 | biostudies-literature | 2009 May

REPOSITORIES: biostudies-literature